Human Dopa Decarboxylase/DCC ELISA Kit (ab277451)
Key features and details
- Sensitivity: 34 pg/ml
- Range: 32.77 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Dopa Decarboxylase/DCC ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
34 pg/ml -
Range
32.77 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 110.6 76% - 145% Plasma 83.28 68% - 108% Cell culture media 128.8 108% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Dopa Decarboxylase/DCC ELISA Kit (ab277451) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Dopa Decarboxylase/DCC in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Dopa Decarboxylase/DCC coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Dopa Decarboxylase/DCC present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Dopa Decarboxylase/DCC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Dopa Decarboxylase/DCC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 350X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Dopa Decarboxylase/DCC Antibody 2 vials Anti-Human Dopa Decarboxylase DCC coated Microplate 1 unit Human Dopa Decarboxylase/DCC standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. -
Involvement in disease
Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Alternative names
- AADC
- Aromatic L Amino Acid Decarboxylase
- Aromatic-L-amino-acid decarboxylase
see all -
Database links
- Entrez Gene: 1644 Human
- Omim: 107930 Human
- SwissProt: P20711 Human
- Unigene: 359698 Human
Images
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Example data
