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Human Dopa Decarboxylase/DCC ELISA Kit (ab277451)

Key features and details

  • Sensitivity: 34 pg/ml
  • Range: 32.77 pg/ml - 8000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Dopa Decarboxylase/DCC ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    34 pg/ml

  • Range

    32.77 pg/ml - 8000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 110.6 76% - 145%
    Plasma 83.28 68% - 108%
    Cell culture media 128.8 108% - 143%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Dopa Decarboxylase/DCC ELISA Kit (ab277451) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Dopa Decarboxylase/DCC in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human Dopa Decarboxylase/DCC coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Dopa Decarboxylase/DCC present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Dopa Decarboxylase/DCC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Dopa Decarboxylase/DCC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    350X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated Anti-Human Dopa Decarboxylase/DCC Antibody 2 vials
    Anti-Human Dopa Decarboxylase DCC coated Microplate 1 unit
    Human Dopa Decarboxylase/DCC standard protein (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.

  • Involvement in disease

    Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.

  • Sequence similarities

    Belongs to the group II decarboxylase family.
  • Information by UniProt

  • Alternative names

    • AADC
    • Aromatic L Amino Acid Decarboxylase
    • Aromatic-L-amino-acid decarboxylase
    • DDC
    • DDC_HUMAN
    • DOPA decarboxylase
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase)
    see all

  • Database links

    Images

    • Example data
      Example data

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