Anti-GCH1 antibody [EPR24155-33] (ab259882)
![Anti-GCH1 antibody [EPR24155-33] (ab259882)](https://www.abcam.com/ps/products/259/ab259882/Images/ab259882-1-anti-gch1-antibody-epr2415533-immunohistochemistry-rat-brain.jpg "Anti-GCH1 antibody [EPR24155-33] (ab259882)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24155-33] to GCH1
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Rat
Overview
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Product name
Anti-GCH1 antibody [EPR24155-33]
See all GCH1 primary antibodies -
Description
Rabbit monoclonal [EPR24155-33] to GCH1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt (Intra)more details
Unsuitable for: ICC,IHC-Fr,IP or WB -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat brain and stomach tissues. Flow Cyt (intra): Rat primary neuron and glia cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24155-33 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] (ab259882)
Immunohistochemical analysis of paraffin-embedded Rat brain tissue labelling GCH1 with ab259882 at 1/500 (1.278 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ventral tegmental area of rat brain. The section was incubated with ab259882 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] (ab259882)](https://www.abcam.com/ps/products/259/ab259882/Images/ab259882-2-anti-gch1-antibody-epr2415533-immunohistochemistry-rat-brain1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] (ab259882)Immunohistochemical analysis of paraffin-embedded Rat brain tissue labelling GCH1 with ab259882 at 1/500 (1.278 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ventral tegmental area of rat brain. The section was incubated with ab259882 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] (ab259882)](https://www.abcam.com/ps/products/259/ab259882/Images/ab259882-3-anti-gch1-antibody-epr2415533-immunohistochemistry-rat-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR24155-33] (ab259882)Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labelling GCH1 with ab259882 at 1/500 (1.278 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat stomach. The section was incubated with ab259882 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Flow Cytometry - Anti-GCH1 antibody [EPR24155-33] (ab259882)](https://www.abcam.com/ps/products/259/ab259882/Images/ab259882-4-anti-gch1-antibody-epr2415533-flow-cytometry-rat-primary-neuron-and-glia-cells.jpg)
Flow Cytometry - Anti-GCH1 antibody [EPR24155-33] (ab259882)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron and glia cells cells labelling GCH1 with ab259882 at 1/500 dilution (0.1ug). Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-GCH1 antibody [EPR24155-33] (ab259882)](https://www.abcam.com/ps/products/259/ab259882/Images/ab259882-5-Benefits-of-Recombinant-antibodies.jpg)
Anti-GCH1 antibody [EPR24155-33] (ab259882)
