Anti-GAPDH antibody (ab263962)
Key features and details
- Rabbit polyclonal to GAPDH
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GAPDH antibody
See all GAPDH primary antibodies -
Description
Rabbit polyclonal to GAPDH -
Host species
Rabbit -
Specificity
Broad reactivity makes this antibody an excellent loading control.
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Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human GAPDH (N terminal).
Database link: P04406 -
Positive control
- WB: NIH/3T3, HEK-293, Molt-4, A549, Jurkat and HeLa lysate. HeLa nuclear lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAPDH antibody (ab263962)All lanes : Anti-GAPDH antibody (ab263962) at 1/1000 dilution (overnight at 4degC)
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) lysate
Lane 3 : Molt-4 (human lymphoblastic leukemia cell line) lysate
Lane 4 : A549 (human lung carcinoma cell line) lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Lane 6 : NIH/3T3 nuclear lysate
Lane 7 : HeLa nuclear lysate
Lane 8 : Jurkat (human T cell leukemia cell line from peripheral blood) lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Peroxidase rabbit secondary antibody for 30 min at RT at 1/70000 dilution
Predicted band size: 36 kDa
