Anti-GABARAP+GABARAPL1+GABARAPL3 antibody [EPR18862] - BSA and Azide free (ab251100)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18862] to GABARAP+GABARAPL1+GABARAPL3 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GABARAP+GABARAPL1+GABARAPL3 antibody [EPR18862] - BSA and Azide free
See all GABARAP+GABARAPL1+GABARAPL3 primary antibodies -
Description
Rabbit monoclonal [EPR18862] to GABARAP+GABARAPL1+GABARAPL3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GABARAP+GABARAPL1+GABARAPL3 aa 1-100. The exact sequence is proprietary.
Database link: Q9H0R8 -
General notes
Ab251100 is the carrier-free version of ab191888. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251100 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR18862 -
Isotype
IgG -
Research areas
Images
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Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution + Human GABARAPL1 full length recombinant protein at 0.02 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Exposure time: 5 secondsThis data was developed using ab191888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human GABARAPL1 full length recombinant protein contains aa1-117 with a GST-Tag.
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All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/5000 dilution
Lane 1 : Recombinant human GABARAPL2 full length protein
Lane 2 : Recombinant human GABARAPL3 full length protein
Lane 3 : Recombinant human GABARAP full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDaThis data was developed using ab191888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 5 seconds; Lane 3: 3 minutes.
All three recombinant human full length proteins contain aa1-117 with an N-terminal His-Tag®. This product showed higher reactivity with GABARAPL1 and GABARAPL3, and lower reactivity with GABARAP.
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Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution + Human brain tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab191888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615; 21862879).
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All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab191888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615; 21862879).
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All lanes : Anti-GABARAP + GABARAPL1 + GABARAPL3 antibody [EPR18862] (ab191888) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab191888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is a little higher than predicted one (14KD), which is similar with the published literatures (PMID: 16650615; 21862879).
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This data was developed using ab191888, the same antibody clone in a different buffer formulation.GABARAP + GABARAPL1 + GABARAPL3 was immunoprecipitated from 1 mg of Mouse brain lysate with ab191888 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab191888 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Mouse brain lysate, 10µg (Input). Lane 2: ab191888 IP in Mouse brain lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab191888 in Mouse brain lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
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