Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FYB antibody [EP2546Y] (ab76103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2546Y] to FYB
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-FYB antibody [EP2546Y]
    See all FYB primary antibodies

  • Description

    Rabbit monoclonal [EP2546Y] to FYB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human FYB aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: O15117

  • Positive control

    • WB: TF-1 lysate. IHC-P: Human tonsil tissue. Flow Cyt: TF-1 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP2546Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-FYB antibody [EP2546Y] (ab76103)
    Western blot - Anti-FYB antibody [EP2546Y] (ab76103)
    Anti-FYB antibody [EP2546Y] (ab76103) at 1/10000 dilution + TF-1 lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] (ab76103)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FYB antibody [EP2546Y] (ab76103)

    ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-FYB antibody [EP2546Y] (ab76103)
    Flow Cytometry - Anti-FYB antibody [EP2546Y] (ab76103)
    Flow Cytometry analysis of TF-1 (Human Erythroleukemia erythroblast) cells labeling FYB with purified ab76103 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Anti-FYB antibody [EP2546Y] (ab76103)
    Anti-FYB antibody [EP2546Y] (ab76103)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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