Anti-Amyloid Precursor Protein antibody [14D6] (ab252814)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [14D6] to Amyloid Precursor Protein
- Suitable for: ELISA, WB
- Reacts with: Rat, Human, Recombinant fragment
Overview
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Product name
Anti-Amyloid Precursor Protein antibody [14D6]
See all Amyloid Precursor Protein primary antibodies -
Description
Rat monoclonal [14D6] to Amyloid Precursor Protein -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB RatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and rat hippocampus tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
14D6 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-Amyloid Precursor Protein antibody [14D6] (ab252814) at 2.308 µg/ml
Lane 1 : Human hippocampus tissue lysate
Lane 2 : Rat hippocampus tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Samples are non-boiled as boiling may cause protein aggregates.
Exposure times: Lane 1: 15 secs; Lane 2: 92 secs.
Blocking/Dilution buffer: 5% NFDM/TBST.
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ELISA - Anti-Amyloid Precursor Protein antibody [14D6] (ab252814).
ab252814 used at 0-4000 ng/ml.
Antigen (Human Amyloid Precursor Protein) used at 1000 ng/ml.
Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
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