Mouse SAP ELISA Kit (ab235639)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.3 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SAP ELISA Kit
See all Serum Amyloid P/SAP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 5 2.4% Inter-assay Sample n Mean SD CV% Mouse serum 3 5.7% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.3 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118 109% - 124% Cell culture media 98 95% - 102% EDTA Plasma 109 95% - 122% Cit plasma 97 83% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse SAP ELISA Kit (ab235639) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SAP protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SAP with 11.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse SAP Capture Antibody 1 x 600µl 10X Mouse SAP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 5BR 1 x 6ml Denaturant 1 x 500µl Mouse SAP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. -
Tissue specificity
Found in serum and urine. -
Involvement in disease
Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Post-translational
modificationsN-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 9.5S alpha 1 glycoprotein
- 9.5S alpha-1-glycoprotein
- Amyloid P component serum
see all -
Database links
- Entrez Gene: 20219 Mouse
- SwissProt: P12246 Mouse
- Unigene: 330510 Mouse
Images
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Other - Mouse SAP ELISA Kit (ab235639)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Leading Competitor ComparisonStandard curve comparison between Mouse SAP SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse SAP standard curve in Sample Diluent NS + Enhancer.The SAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SAP in mouse serum and plasma samples.The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100,000, plasma (citrate) 1:100,000, plasma (EDTA) 1:100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 197 µg/mL in neat serum, 98.7 µg/mL in neat plasma (citrate) and 176 µg/mL in neat plasma (EDTA).
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Interpolated concentrations of native SAP in mouse kidney and lung tissue supernatant samples.The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse kidney tissue supernatant 1:100 and mouse lung tissue supernatant 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 43 ng/mL in neat kidney tissue supernatant and 113 ng/mL in neat lung tissue supernatant.
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Serum samples from three strains of mice were measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 193 µg/mL in neat CD-1 serum, 433 µg/mL in neat C57BL-6 serum, and 583 µg/mL in neat BALB/c serum.
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Sandwich ELISA - Mouse SAP ELISA Kit (ab235639)To learn more about the advantages of recombinant antibodies see here.