Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)
![Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)](https://www.abcam.com/ps/products/251/ab251062/Images/ab251062-405524-anti-fxyd1-plm-antibody-epr16081-bsa-and-azide-free-western-blot.jpg "Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16081] to FXYD1/PLM - BSA and Azide free
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free
See all FXYD1/PLM primary antibodies -
Description
Rabbit monoclonal [EPR16081] to FXYD1/PLM - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251062 is the carrier-free version of ab191397.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16081 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)All lanes : Anti-FXYD1/PLM antibody [EPR16081] (ab191397) at 1/10000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 10 kDaThis data was developed using ab191397, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)](https://www.abcam.com/ps/products/251/ab251062/Images/ab251062-2-anti-fxyd1-plm-antibody-epr16081-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)This data was developed using ab191397, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling FXYD1/PLM with ab191397 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)](https://www.abcam.com/ps/products/251/ab251062/Images/ab251062-1-anti-fxyd1-plm-antibody-epr16081-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)This data was developed using ab191397, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal livert lysate immunoprecipitated using ab191397 at 1/90 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
![Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)](https://www.abcam.com/ps/products/251/ab251062/Images/ab251062-4-benefits-of-recombinant-antibodies.png)
Anti-FXYD1/PLM antibody [EPR16081] - BSA and Azide free (ab251062)
