Anti-FOXP3 antibody (ab10901)
Key features and details
- Rabbit polyclonal to FOXP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXP3 antibody
See all FOXP3 primary antibodies -
Description
Rabbit polyclonal to FOXP3 -
Host species
Rabbit -
Specificity
This antibody recognises a single clean band representing FOXP3 on HEK293 cell lysate overexpressing human FOXP3. This band is blocked by the immunising peptide and is not present in the control HEK293 cell lysate. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab16809) -
Positive control
- This antibody gave a positive signal in Hek 293 lysate over expressing human FOXP3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Notes WB
Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Target
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Function
Probable transcription factor. Plays a critical role in the control of immune response. -
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Images
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Western blot - Anti-FOXP3 antibody (ab10901)
Western blot using ab10901 at 1/500.
Lane 1: HEK 293 lysate.
A no primary control lane showed no bands.
Lane 2: HEK 293 lysate over expressing human FOXP3.
Lane 3: HEK 293 lysate over expressing mouse FOXP3.
Lane 4: HEK 293 lysate with FOXP3 peptide ab16809.
Lane 5: HEK 293 lysate over expressing human FOXP3 with FOXP3 peptide ab16809.
Lane 6: HEK 293 lysate over expressing mouse FOXP3 with FOXP3 peptide ab16809.
All lanes with ab10901.
A no primary control lane showed no bands.
Peptide at 1µ
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 1 minute.
Expected molecular weight: 47kD
Lysates at 20µ g/lane.
Datasheets and documents
References (11)
ab10901 has been referenced in 11 publications.
- Ma F et al. Melatonin ameliorates necrotizing enterocolitis by preventing Th17/Treg imbalance through activation of the AMPK/SIRT1 pathway. Theranostics 10:7730-7746 (2020). PubMed: 32685016
- Skretting G et al. Transcription factor FOXP3: A repressor of the TFPI gene? J Cell Biochem N/A:N/A (2019). PubMed: 30861202
- Ma F et al. Interleukin-6-mediated CCR9+ interleukin-17-producing regulatory T cells polarization increases the severity of necrotizing enterocolitis. EBioMedicine 44:71-85 (2019). PubMed: 31129099
- Zeng C et al. Downregulation of FOXP3 in neutrophils by IL-8 promotes the progression of oral squamous cell carcinoma. Oncol Lett 18:4771-4777 (2019). PubMed: 31611987
- Ma H et al. STAT5 and TET2 Cooperate to Regulate FOXP3-TSDR Demethylation in CD4+ T Cells of Patients with Colorectal Cancer. J Immunol Res 2018:6985031 (2018). PubMed: 30013992
Images
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Western blot - Anti-FOXP3 antibody (ab10901)
Western blot using ab10901 at 1/500.
Lane 1: HEK 293 lysate.
A no primary control lane showed no bands.
Lane 2: HEK 293 lysate over expressing human FOXP3.
Lane 3: HEK 293 lysate over expressing mouse FOXP3.
Lane 4: HEK 293 lysate with FOXP3 peptide ab16809.
Lane 5: HEK 293 lysate over expressing human FOXP3 with FOXP3 peptide ab16809.
Lane 6: HEK 293 lysate over expressing mouse FOXP3 with FOXP3 peptide ab16809.
All lanes with ab10901.
A no primary control lane showed no bands.
Peptide at 1µ
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 1 minute.
Expected molecular weight: 47kD
Lysates at 20µ g/lane.
