Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [221D] to FOXP3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXP3 antibody [221D] - BSA and Azide free
    See all FOXP3 primary antibodies

  • Description

    Mouse monoclonal [221D] to FOXP3 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate. IHC-P: Human stomach carcinoma, tonsil and colon tissue; Mouse spleen and thymus tissue.

  • General notes

    ab255942 is the carrier-free version of ab253297. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Western blot - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    All lanes : Anti-FOXP3 antibody [221D] (ab253297) at 1.514 µg/ml

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-myc tag, whole cell lysate
    Lane 2 : HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 47 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    The molecular weight observed was in consistant with the literature (PMID: 17586580).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253297).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the human tonsil. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253297).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the stroma cells in the human colon. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253297).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

    Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the stroma cells in the human stomach carcinoma. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253297).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on mouse spleen tissue. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253297).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on mouse thymus tissue. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253297).

  • Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)
    Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

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