Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7441(2)] to FKBP38 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free
    See all FKBP38 primary antibodies

  • Description

    Rabbit monoclonal [EPR7441(2)] to FKBP38 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248311 is the carrier-free version of ab129113 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248311 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
    Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

    This data was developed using ab129113, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: ProteinX knockout HAP1 cell lysate (20 µg) 

    Lane 3: CellX1 cell lysate (20 µg)

    Lane 4: CellX2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab129113 observed at 51 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab129113 was shown to specifically react with FKBP38 when FKBP38 knockout samples were used. Wild-type and FKBP38 knockout samples were subjected to SDS-PAGE. ab129113 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
    Western blot - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
    All lanes : Anti-FKBP38 antibody [EPR7441(2)] (ab129113) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab129113, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
    Immunocytochemistry - Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
    This data was developed using ab129113, the same antibody clone in a different buffer formulation.ab129113, at 1/100 dilution, staining FKBP38 in HeLa cells by Immunofluorescence.
  • Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)
    Anti-FKBP38 antibody [EPR7441(2)] - BSA and Azide free (ab248311)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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