anti-Filgrastim ELISA Kit (Neupogen®) (ab237668)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3.1 ng/ml - 50 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
anti-Filgrastim ELISA Kit (Neupogen®)
See all G-CSF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 ng/ml -
Range
3.1 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
anti-Filgrastim ELISA Kit (Neupogen®) (ab237668) is a highly specific and sensitive kit designed for the in vitro determination of antibody against Filgrastim in biological matrices such as human serum and plasma. The density of color is proportional to the amount of human antibody against Filgrastim captured from the samples.
Filgrastim (Neupogen®) is a recombinant, non-pegylated human granulocyte colony stimulating factor (G-CSF) analogue. It binds to the GCSF receptor and stimulates the production of neutrophils in the bone marrow. As a G-CSF analog, it controls proliferation of committed progenitor cells and influences their maturation into mature neutrophils. Filgrastim is used in patients with acute myeloid leukemia receiving induction or consolidation chemotherapy. It is also used in cancer patients receiving bone marrow transplant. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 2 x 50ml Confirmation Reagent 1 x 1ml Filgrastim Standard S1 1 x 0.3ml Filgrastim Standard S2 1 x 0.3ml Filgrastim Standard S3 1 x 0.3ml Filgrastim Standard S4 1 x 0.3ml Filgrastim Standard S5 1 x 0.3ml Filgrastim Standard S6 1 x 0.3ml Filgrastim Standard S7 1 x 0.3ml Filgrastim Standard S8 1 x 0.3ml Micro ELISA Plate 1 unit Peroxidase Conjugate 1 x 12ml Plate sealers 2 units Stop Solution 1 x 12ml STREP-HRP 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Research areas
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C17orf33
- Colony stimulating factor 3
- Colony stimulating factor 3 (granulocyte)
see all -
Database links
- Entrez Gene: 1440 Human
- Omim: 138970 Human
- SwissProt: P09919 Human
- Unigene: 2233 Human
Images
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Example Data
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.