Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
![Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288797-anti-fibrinogen-antibody-epr18145-84-western-blot.jpg "Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Fibrinogen beta chain antibody [EPR18145-84]
See all Fibrinogen beta chain primary antibodies -
Description
Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver tissue lysate and plasma; rat liver, platelets and plasma; mouse liver and plasma; HepG2 and Hepa1-6 whole cell lysate. IHC-P: Mouse liver and spleen tissue; rat liver and spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18145-84 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human plasma
Lane 3 : Rat plasma
Lane 4 : Rat platelet lysate
Lane 5 : Mouse plasma
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lanes 1-4: 3 seconds
Lane 5: 2 seconds
Lane 6: 1 minute
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![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288798-anti-fibrinogen-antibody-epr18145-84-western-blot.jpg)
Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Rat liver lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lane 1: 3 seconds
Lane 2: 1 second
Lane 3: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288799-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288800-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288801-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288802-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-7-benefits-of-recombinant-antibodies.png)
Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
