Anti-FGF9/GAF antibody [EPR19937] (ab206408)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19937] to FGF9/GAF
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-FGF9/GAF antibody [EPR19937]
See all FGF9/GAF primary antibodies -
Description
Rabbit monoclonal [EPR19937] to FGF9/GAF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human FGF9/GAF active protein; human fetal brain, fetal kidney and ovary cancer lysates; mouse kidney and brain lysates; rat brain lysate; C6, PC-12, NIH/3T3 and U-87 MG whole cell lysates.
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General notes
Previously labelled as FGF9.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19937 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1 : Human FGF9/GAF active protein
Lane 2 : Human FGF16 recombinant protein
Lane 3 : Human FGF20 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 1 seconds; Lane 3: 15 seconds.
Human FGF9/GAF active protein contains aa1-208; FGF16 and FGF20 recombinant protein contain aa1-203 and aa4-208 respectively, both with His-Tag®.
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All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.
The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).
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All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.
The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).
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All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 15 seconds; Lane 4: 3 minutes.
The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).
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