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Signal Transduction Growth Factors/Hormones FGF

Anti-FGF9/GAF antibody [EPR19937] (ab206408)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-FGF9/GAF antibody [EPR19937] (ab206408)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19937] to FGF9/GAF
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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Overview

  • Product name

    Anti-FGF9/GAF antibody [EPR19937]
    See all FGF9/GAF primary antibodies
  • Description

    Rabbit monoclonal [EPR19937] to FGF9/GAF
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human FGF9/GAF active protein; human fetal brain, fetal kidney and ovary cancer lysates; mouse kidney and brain lysates; rat brain lysate; C6, PC-12, NIH/3T3 and U-87 MG whole cell lysates.
  • General notes

    Previously labelled as FGF9.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19937
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • FGF
    • FGF
    • Signal Transduction
    • Growth Factors/Hormones
    • FGF
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Reproduction
    • Sex determination
    • Developmental Biology
    • Reproduction
    • Placental development

Images

  • Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : Human FGF9/GAF active protein
    Lane 2 : Human FGF16 recombinant protein
    Lane 3 : Human FGF20 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 1 seconds; Lane 3: 15 seconds.

    Human FGF9/GAF active protein contains aa1-208; FGF16 and FGF20 recombinant protein contain aa1-203 and aa4-208 respectively, both with His-Tag®. 

  • Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.

    The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).

  • Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 15 seconds; Lane 3: 30 seconds.

    The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).

  • Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    Western blot - Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-3: 15 seconds; Lane 4: 3 minutes.

    The MW observed is consistent with the literature (PMID:16452189, PMID: 25925261).

  • Anti-FGF9/GAF antibody [EPR19937] (ab206408)
    Anti-FGF9/GAF antibody [EPR19937] (ab206408)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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