Anti-FGF22 antibody (ab74860)
Key features and details
- Rabbit polyclonal to FGF22
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF22 antibody -
Description
Rabbit polyclonal to FGF22 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide derived from internal sequence of human FGF22.
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Positive control
- Human ovary tissue. Extracts from Jurkat cells and HT-29 cells. MCF-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FGF22 antibody (ab74860)All lanes : Anti-FGF22 antibody (ab74860) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF22 antibody (ab74860)Immunohistochemistry analysis of FGF22 in paraffin-embedded human ovary tissue using ab74860, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-FGF22 antibody (ab74860)Immunofluorescence analysis of FGF22 in MCF-7 cells using ab74860, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
