Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF 23 antibody (ab98000)

Key features and details

  • Rabbit polyclonal to FGF 23
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FGF 23 antibody
    See all FGF 23 primary antibodies

  • Description

    Rabbit polyclonal to FGF 23

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human heart; Human bone marrow; Human bone tumour as well as the following whole cell lysates: U2OS; U937; MOLT4.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab98000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.

  • Tissue specificity

    Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).

  • Involvement in disease

    Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
    Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues.

  • Sequence similarities

    Belongs to the heparin-binding growth factors family.

  • Post-translational
    modifications

    Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
    O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23.

  • Cellular localization

    Secreted. Secretion is dependent on O-glycosylation.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADHR antibody
      • FGF-23 antibody
      • Fgf23 antibody
      • FGF23_HUMAN antibody
      • FGFN antibody
      • Fibroblast growth factor 23 antibody
      • Fibroblast growth factor 23 C-terminal peptide antibody
      • Fibroblast growth factor 23 precursor antibody
      • HPDR2 antibody
      • HYPF antibody
      • Phosphatonin antibody
      • PHPTC antibody
      • Tumor derived hypophosphatemia inducing factor antibody
      • Tumor-derived hypophosphatemia-inducing factor antibody
      see all

    Images

    • Western blot - Anti-FGF 23 antibody (ab98000)
      Western blot - Anti-FGF 23 antibody (ab98000)
      All lanes : Anti-FGF 23 antibody (ab98000) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
      Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
      Lane 4 : Human bone tumor tissue lysate - total protein (ab29359)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 27 kDa
      Observed band size: 27 kDa
      Additional bands at: 48 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      This antibody has also given a positive signal in Human heart tissue lysate and U2OS and U937 whole cell lysates.

    References (3)

    Publishing research using ab98000? Please let us know so that we can cite the reference in this datasheet.

    ab98000 has been referenced in 3 publications.

    • Rodrat M  et al. Prolonged exposure to 1,25(OH)2D3 and high ionized calcium induces FGF-23 production in intestinal epithelium-like Caco-2 monolayer: A local negative feedback for preventing excessive calcium transport. Arch Biochem Biophys 640:10-16 (2018). WB . PubMed: 29317227
    • Leifheit-Nestler M  et al. Impact of Altered Mineral Metabolism on Pathological Cardiac Remodeling in Elevated Fibroblast Growth Factor 23. Front Endocrinol (Lausanne) 9:333 (2018). PubMed: 29977226
    • Wongdee K  et al. Activation of calcium-sensing receptor by allosteric agonists cinacalcet and AC-265347 abolishes the 1,25(OH)2D3-induced Ca2+ transport: Evidence that explains how the intestine prevents excessive Ca2+ absorption. Arch Biochem Biophys 657:15-22 (2018). PubMed: 30217510

    Images

    • Western blot - Anti-FGF 23 antibody (ab98000)
      Western blot - Anti-FGF 23 antibody (ab98000)
      All lanes : Anti-FGF 23 antibody (ab98000) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
      Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
      Lane 4 : Human bone tumor tissue lysate - total protein (ab29359)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 27 kDa
      Observed band size: 27 kDa
      Additional bands at: 48 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      This antibody has also given a positive signal in Human heart tissue lysate and U2OS and U937 whole cell lysates.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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