Antibodies, Proteins, Kits and Reagents for Life Science

465 705 ₸ Product size

1 ml 465 705 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP237] to Fbxw7
Suitable for: Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-Fbxw7 antibody [SP237]
See all Fbxw7 primary antibodies
Description

Rabbit monoclonal [SP237] to Fbxw7
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

IHC-P
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human endometrial carcinoma, and Human endometrial adenocarcinoma tissue; Flow Cyt: HL-60 cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Application	Species
Flow Cyt	Human
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP237
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] (ab227677)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial adenocarcinoma tissue sections labeling Fbxw7 with ab227677 at 1/100 dilution (1.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-Fbxw7 antibody [SP237] (ab227677)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Fbxw7 with ab227677 at 1/400 (green) compared with a rabbit isotype control (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] (ab227677)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Fbxw7 with ab227677 at 1/100 dilution in immunohistochemical analysis.
Anti-Fbxw7 antibody [SP237] (ab227677)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com