Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2279(3)] to FANCD2
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-FANCD2 antibody [EPR2279(3)]
See all FANCD2 primary antibodies
Description

Rabbit monoclonal [EPR2279(3)] to FANCD2
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FANCD2 aa 1400 to the C-terminus (Cysteine residue). The exact sequence is proprietary. (Phospho specific peptide).
Database link: Q9BXW9
Positive control
- WB: HAP1 and HeLa whole cell lysate (ab150035). Flow Cyt: HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2279(3)
Isotype

IgG
Research areas

Western blot - Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

All lanes : Anti-FANCD2 antibody [EPR2279(3)] (ab178705) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FANCD2 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 166 kDa
Observed band size: 166 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab178705 observed at 166 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab178705 was shown to react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type HeLa and FANCD2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178705 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

All lanes : Anti-FANCD2 antibody [EPR2279(3)] (ab178705) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : FANCD2 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HEK293 cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 166 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab178705 observed at 166 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab178705 was shown to specifically react with FANCD2 when FANCD2 knockout samples were used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. ab178705 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Flow Cytometry - Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

Flow cytometric analysis of permeabilized HeLa cells labeling FANCD2 with ab178705 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Western blot - Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

All lanes : Anti-FANCD2 antibody [EPR2279(3)] (ab178705) at 1/1000 dilution

Lane 1 : HeLa cell lysate with FANCD2 (pS1418) peptide
Lane 2 : HeLa cell lysate with Non-phospho peptide
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 166 kDa
Western blot - Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

All lanes : Anti-FANCD2 antibody [EPR2279(3)] (ab178705) at 1/1000 dilution

Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase
Lane 3 : HeLa cell lysate treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Predicted band size: 166 kDa
Anti-FANCD2 antibody [EPR2279(3)] (ab178705)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com