Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)
![Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)](https://www.abcam.com/ps/products/248/ab248236/Images/ab248236-405500-anti-cps1-antibody-epr7494-bsa-and-azide-free-western-blot.jpg "Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7494] to CPS1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-CPS1 antibody [EPR7494] - BSA and Azide free
See all CPS1 primary antibodies -
Description
Rabbit monoclonal [EPR7494] to CPS1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248236 is the carrier-free version of ab128942 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248236 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7494 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)All lanes : Anti-CPS1 antibody [EPR7494] (ab128942) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 165 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab128942, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)](https://www.abcam.com/ps/products/248/ab248236/Images/ab248236-2-anti-cps1-antibody-epr7494-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)This data was developed using ab128942, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPS1 with unpurified ab128942 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)](https://www.abcam.com/ps/products/248/ab248236/Images/ab248236-1-anti-cps1-antibody-epr7494-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)This data was developed using ab128942, the same antibody clone in a different buffer formulation.ab128942, at 1/250 dilution, staining CPS1 in paraffin-embedded Human liver tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)](https://www.abcam.com/ps/products/248/ab248236/Images/ab248236-4-benefits-of-recombinant-antibodies.png)
Anti-CPS1 antibody [EPR7494] - BSA and Azide free (ab248236)