Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16519-42] to FANCA/FAA - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free
    See all FANCA/FAA primary antibodies

  • Description

    Rabbit monoclonal [EPR16519-42] to FANCA/FAA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251345 is the carrier-free version of ab201458 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251345 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as FANCA

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)
    Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)

    This data was developed using ab201458, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 µg)

    Lane 2 ProteinX knockout HAP1 cell lysate (20 µg)

    Lane 3 HeLa cell lysate (20 µg)

    Lane 4 Jurkat cell lysate (20 µg)

    Lanes 1 - 4 Merged signal (red and green). Green - ab201458 observed at 163 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab201458 was shown to recognize FANCA/FAA when FANCA/FAA knockout samples were used, along with additional cross-reactive bands. Wild-type and FANCA/FAA knockout samples were subjected to SDS-PAGE. ab201458 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)
    Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)
    All lanes : Anti-FANCA/FAA antibody [EPR16519-42] (ab201458) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293 (Human embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 163 kDa
    Observed band size: 163 kDa


    Exposure time: 1 minute


    This data was developed using ab201458, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)
    Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)
    All lanes : Anti-FANCA/FAA antibody [EPR16519-42] (ab201458) at 1/1000 dilution

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 163 kDa
    Observed band size: 163 kDa


    Exposure time: 3 minutes


    This data was developed using ab201458, the same antibody clone in a different buffer formulation.

  • Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)
    Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (ab251345)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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