Anti-FANCA/FAA antibody (ab264257)
Key features and details
- Rabbit polyclonal to FANCA/FAA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCA/FAA antibody
See all FANCA/FAA primary antibodies -
Description
Rabbit polyclonal to FANCA/FAA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human FANCA/FAA aa 1405-1455. The exact sequence is proprietary.
Database link: O15360 -
Positive control
- IP: HeLa whole cell lysate; WB: HeLa, K-562, A-549, MCF-7 and HEK-293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FANCA/FAA antibody (ab264257)All lanes : Anti-FANCA/FAA antibody (ab264257) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K-562 whole cell lysate
Lane 3 : A-549 whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lane 5 : HEK-293T whole cell lysate
Lysates/proteins at 50 µg per lane.Detected with chemilimuniscence with exposure time of 30 seconds.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-FANCA/FAA antibody (ab264257)FANCA/FAA was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264257 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264257 at 0.04 µg/ml.
Lane 1: ab264257 IP in HeLa whole cell lysate.
Lane 2: ab264257 IP in HeLa whole cell lysate.
Lane 3: Control IgG.Exposure time: 75 secs.
Different lots were used in lane 1 and lane 2.
Cells prepared using NETN lysis buffer.
