Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23948-48] to Factor I/CFI
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor I/CFI antibody [EPR23948-48]
    See all Factor I/CFI primary antibodies

  • Description

    Rabbit monoclonal [EPR23948-48] to Factor I/CFI

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Human liver, lung, small intestine and plasma; Human liver treated with PNGase F and HepG2 treated with PNGase F. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23948-48

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)
    Western blot - Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)
    All lanes : Anti-Factor I/CFI antibody [EPR23948-48] (ab278524) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 2 : A549 (human lu carcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 3 : Human liver tissue lysate at 40 µg
    Lane 4 : Human lung tissue lysate at 40 µg
    Lane 5 : Human small intestine tissue lysate at 40 µg
    Lane 6 : Human plasma at 10 µl

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 66 kDa
    Observed band size: 88/50 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17597211).

    Low expression: A549 (PMID:17548110).

    Exposure time: Lanes 1-3: 15 seconds; Lanes 4-6: 5.5 seconds.

     

  • Western blot - Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)
    Western blot - Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)
    All lanes : Anti-Factor I/CFI antibody [EPR23948-48] (ab278524) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate at 20 µg
    Lane 2 : Human liver tissue lysate treated with PNGase F at 20 µg
    Lane 3 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate 20
    Lane 4 : HepG2 whole cell lysate treated with PNGase F at 20 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 66 kDa
    Observed band size: 50,40,88,75 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Factor I/CFI is a glycoprotein, and de-glycosylation resulted in a decrease of MW (lanes 2 and 4). Different band sizes of CFI heavy chain in liver and HepG2 lysates (lanes 1 and 3) observed may be caused by different glycosylation levels.

    Exposure time: 26 seconds.

     

  • Immunoprecipitation - Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)
    Immunoprecipitation - Anti-Factor I/CFI antibody [EPR23948-48] (ab278524)

    Factor I/CFI was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab278524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab278524 IP in HepG2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of abid in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds

     

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