Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)
![Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)](https://www.abcam.com/ps/products/251/ab251417/Images/ab251417-403393-anti-factor-d-cfd-antibody-epr14699-bsa-and-azide-free-western-blot.jpg "Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14699] to Factor D/CFD - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free
See all Factor D/CFD primary antibodies -
Description
Rabbit monoclonal [EPR14699] to Factor D/CFD - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251417 is the carrier-free version of ab204917 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251417 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Factor D
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR14699 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)All lanes : Anti-Factor D/CFD antibody [EPR14699] (ab204917) at 1/2000 dilution
Lane 1 : Human serum
Lane 2 : THP-1 (Human monocytic leukemia cells) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab204917, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)](https://www.abcam.com/ps/products/251/ab251417/Images/ab251417-399202-anti-factor-d-cfd-antibody-epr14699-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)All lanes : Anti-Factor D/CFD antibody [EPR14699] (ab204917) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : THP-1 (Human monocytic leukemia cells) cell lysate
Lane 3 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : Molt4 (Human lymphoblastic leukemia cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab204917, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)](https://www.abcam.com/ps/products/251/ab251417/Images/ab251417-3-benefits-of-recombinant-antibodies.png)
Anti-Factor D/CFD antibody [EPR14699] - BSA and Azide free (ab251417)