Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)
![Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)](https://www.abcam.com/ps/products/250/ab250478/Images/ab250478-403495-anti-factor-d-cfd-antibody-epr17004-285-bsa-and-azide-free-western-blot.jpg "Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17004-285] to Factor D/CFD - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free
See all Factor D/CFD primary antibodies -
Description
Rabbit monoclonal [EPR17004-285] to Factor D/CFD - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects a distinct epitope compared to ab213177.
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Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250478 is the carrier-free version of ab181307 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250478 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Factor D
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17004-285 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/100000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab181307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)](https://www.abcam.com/ps/products/250/ab250478/Images/ab250478-398717-anti-factor-d-cfd-antibody-epr17004-285-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)All lanes : Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse serum lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?This data was developed using ab181307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 1 second; Lane 3: 15 seconds.
The higher molecular mass corresponds to glycosylated forms of the protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; 12949072).
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![Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)](https://www.abcam.com/ps/products/250/ab250478/Images/ab250478-3-benefits-of-recombinant-antibodies.png)
Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)