Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FABP5 antibody [EPR22552-64] (ab255276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22552-64] to FABP5
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FABP5 antibody [EPR22552-64]
    See all FABP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR22552-64] to FABP5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231, HeLa and PC-3 whole cell lysates; Mouse skin lysate; Rat skin lysate. IHC-P: Human placenta, cerebral cortex and skin tissue. ICC/IF: MDA-MB-231 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human skin (PMID: 8092987) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] (ab255276)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma cell line) and T-47D (human ductal breast epithelial tumor cell line) cells labeling FABP5 with ab255276 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in MDA-MB-231 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PBS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: T-47D (PMID: 21356353).

  • Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    All lanes : Anti-FABP5 antibody [EPR22552-64] (ab255276) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : FABP5 knockout HeLa cell lysate
    Lane 3 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 15 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab255276 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab255276 Anti-FABP5 antibody [EPR22552-64] was shown to specifically react with FABP5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265905 (knockout cell lysate ab257431) was used.  Wild-type and FABP5 knockout samples were subjected to SDS-PAGE.  ab255276 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    All lanes : Anti-FABP5 antibody [EPR22552-64] (ab255276) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell line) whole cell lysate
    Lane 4 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 8 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25260874).

    Negative control: T47D (PMID: 21356353).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cerebral cortex (PMID: 24114376) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on blood vessels of human placenta (PMID: 19625659) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-FABP5 antibody [EPR22552-64] (ab255276)
    Anti-FABP5 antibody [EPR22552-64] (ab255276)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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