Mouse FABP4 ELISA (ab277426)
Key features and details
- Sensitivity: 14 pg/ml
- Range: 12.29 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse FABP4 ELISA
See all FABP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
12.29 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 72.64 69% - 80% Plasma 72.89 67% - 79% Cell culture media 133.1 128% - 139% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse FABP4 ELISA Kit (ab277426) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FABP4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse FABP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse FABP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse FABP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse FABP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Mouse FABP4 Antibody 2 vials Anti-Mouse FABP4 coated Microplate 1 unit Mouse FABP4 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. - Information by UniProt
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Alternative names
- 3T3-L1 lipid-binding protein
- 422/aP2
- A-FABP
see all -
Database links
- Entrez Gene: 11770 Mouse
- SwissProt: P04117 Mouse
- Unigene: 582 Mouse
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.