Anti-FABP4 antibody (ab66682)
Key features and details
- Rabbit polyclonal to FABP4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FABP4 antibody
See all FABP4 primary antibodies -
Description
Rabbit polyclonal to FABP4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human FABP4 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab66681) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Target
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Function
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. - Information by UniProt
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Database links
- Entrez Gene: 2167 Human
- Entrez Gene: 11770 Mouse
- Entrez Gene: 79451 Rat
- Omim: 600434 Human
- SwissProt: P15090 Human
- SwissProt: P04117 Mouse
- SwissProt: P70623 Rat
- Unigene: 391561 Human
see all -
Alternative names
- 3T3-L1 lipid-binding protein antibody
- 422/aP2 antibody
- A-FABP antibody
see all
Images
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Western blot - Anti-FABP4 antibody (ab66682)All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml
Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
Lane 2 : P7 Mouse Adipose Tissue Lysate
Lane 3 : Adult Rat Brown Adipose Tissue Lysate (ab48639)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 30 kDa, 44 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab66682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-FABP4 antibody (ab66682)All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml
Lane 1 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 2 :Mouse thymus tissue lysate - total protein (ab29285)
Lane 3 : Rat Ovary Tissue Lysate
Lane 4 : Rat Colon Tissue Lysate
Lane 5 : Rat Thymus Tissue Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 18 kDa (possible post-translational modification)
Datasheets and documents
References (13)
ab66682 has been referenced in 13 publications.
- Furuhata R et al. Progesterone receptor membrane associated component 1 enhances obesity progression in mice by facilitating lipid accumulation in adipocytes. Commun Biol 3:479 (2020). PubMed: 32887925
- Sun Y et al. miR-142 controls metabolic reprogramming that regulates dendritic cell activation. J Clin Invest 129:2029-2042 (2019). PubMed: 30958798
- Tang W et al. miR-455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide. Mol Med Rep 20:4781-4790 (2019). PubMed: 31638263
- Masia F et al. Label-free quantitative chemical imaging and classification analysis of adipogenesis using mouse embryonic stem cells. J Biophotonics 11:e201700219 (2018). PubMed: 29573183
- Jourdan T et al. Decreasing CB1 receptor signaling in Kupffer cells improves insulin sensitivity in obese mice. Mol Metab 6:1517-1528 (2017). PubMed: 29107297
Images
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Western blot - Anti-FABP4 antibody (ab66682)All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml
Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
Lane 2 : P7 Mouse Adipose Tissue Lysate
Lane 3 : Adult Rat Brown Adipose Tissue Lysate (ab48639)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 30 kDa, 44 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab66682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-FABP4 antibody (ab66682)All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml
Lane 1 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 2 :Mouse thymus tissue lysate - total protein (ab29285)
Lane 3 : Rat Ovary Tissue Lysate
Lane 4 : Rat Colon Tissue Lysate
Lane 5 : Rat Thymus Tissue Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 18 kDa (possible post-translational modification)
