Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FABP4 antibody (ab66682)

Key features and details

  • Rabbit polyclonal to FABP4
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FABP4 antibody
    See all FABP4 primary antibodies

  • Description

    Rabbit polyclonal to FABP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human FABP4 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab66681)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab66682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • Function

    Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

  • Cellular localization

    Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Images

  • Western blot - Anti-FABP4 antibody (ab66682)
    Western blot - Anti-FABP4 antibody (ab66682)
    All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : P7 Mouse Adipose Tissue Lysate
    Lane 3 : Adult Rat Brown Adipose Tissue Lysate (ab48639)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 30 kDa, 44 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab66682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-FABP4 antibody (ab66682)
    Western blot - Anti-FABP4 antibody (ab66682)
    All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 3 : Rat Ovary Tissue Lysate
    Lane 4 : Rat Colon Tissue Lysate
    Lane 5 : Rat Thymus Tissue Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 18 kDa (possible post-translational modification)

References (13)

Publishing research using ab66682? Please let us know so that we can cite the reference in this datasheet.

ab66682 has been referenced in 13 publications.

  • Furuhata R  et al. Progesterone receptor membrane associated component 1 enhances obesity progression in mice by facilitating lipid accumulation in adipocytes. Commun Biol 3:479 (2020). PubMed: 32887925
  • Sun Y  et al. miR-142 controls metabolic reprogramming that regulates dendritic cell activation. J Clin Invest 129:2029-2042 (2019). PubMed: 30958798
  • Tang W  et al. miR-455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide. Mol Med Rep 20:4781-4790 (2019). PubMed: 31638263
  • Masia F  et al. Label-free quantitative chemical imaging and classification analysis of adipogenesis using mouse embryonic stem cells. J Biophotonics 11:e201700219 (2018). PubMed: 29573183
  • Jourdan T  et al. Decreasing CB1 receptor signaling in Kupffer cells improves insulin sensitivity in obese mice. Mol Metab 6:1517-1528 (2017). PubMed: 29107297
View all Publications for this product

Images

  • Western blot - Anti-FABP4 antibody (ab66682)
    Western blot - Anti-FABP4 antibody (ab66682)
    All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : P7 Mouse Adipose Tissue Lysate
    Lane 3 : Adult Rat Brown Adipose Tissue Lysate (ab48639)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 30 kDa, 44 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab66682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Western blot - Anti-FABP4 antibody (ab66682)
    Western blot - Anti-FABP4 antibody (ab66682)
    All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 3 : Rat Ovary Tissue Lysate
    Lane 4 : Rat Colon Tissue Lysate
    Lane 5 : Rat Thymus Tissue Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa
    Additional bands at: 18 kDa (possible post-translational modification)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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