Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3579] to FABP4
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-FABP4 antibody [EPR3579]
See all FABP4 primary antibodies
Description

Rabbit monoclonal [EPR3579] to FABP4
Host species

Rabbit
Specificity

Ab92501 may cross-react with FABP, FABP3 and FABP9 based on the blast alignments.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

A synthetic peptide corresponding to residues in Human FABP4.
Positive control
- WB: Mouse brown adipose tissue, Mouse heart, Mouse kidney, Mouse lung, Human adipose tissue,Rat adipose tissue and fetal heart lysates; ICC/IF: Adipocytes and 3T3-L1 cells; IHC-P: human breast tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3579
Isotype

IgG
Research areas

Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)

All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution (Purified)

Lane 1 : Mouse brown adipose tissue lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse lung lysates
Lane 5 : Human adipose tissue lysates
Lane 6 : Rat adipose tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

FABP4 is abundantly expressed in adipose tissue and at a lower level in lung, heart, skin, kidney, liver and brain (PMID: 23143994).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (ab92501)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FABP4 with Purified ab92501 at 1/16,000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)

Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (Mouse embryonic fibroblast) differentiated for 6 days cells labeling FABP4 with Purifiedab92501 at 1/50 dilution (9.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (ab92501)

FABP4 (green) was detected using FABP4 primary antibody (unpurified ab92501; diluted 1/1000). Alpha tubulin (red) was detected using our mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.
Western blot - Anti-FABP4 antibody [EPR3579] (ab92501)

All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution (unpurified)

Lane 1 : Human adipose
tissue lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa
Anti-FABP4 antibody [EPR3579] (ab92501)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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