Anti-Ezrin antibody (ab231907)
Key features and details
- Rabbit polyclonal to Ezrin
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Ezrin antibody
See all Ezrin primary antibodies -
Description
Rabbit polyclonal to Ezrin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Rabbit, Cow
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Ezrin aa 1-300. N-terminal tags. Expressed in E. coli.
Database link: P15311 -
Positive control
- WB: Recombinant human Ezrin protein; HeLa whole cell lysate; human lung and porcine stomach tissue lysate. IHC-P: Human prostate gland cancer, kidney, stomach, pancreas, breast cancer and prostate cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ezrin antibody (ab231907)All lanes : Anti-Ezrin antibody (ab231907) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Porcine stomach tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 69 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (ab231907)Formalin-fixed, paraffin-embedded human prostate gland cancer tissue stained for Ezrin using ab231907 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (ab231907)Formalin-fixed, paraffin-embedded human kidney tissue stained for Ezrin using ab231907 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (ab231907)Formalin-fixed, paraffin-embedded human stomach tissue stained for Ezrin using ab231907 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (ab231907)Formalin-fixed, paraffin-embedded human pancreas tissue stained for Ezrin using ab231907 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (ab231907)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Ezrin using ab231907 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (ab231907)Formalin-fixed, paraffin-embedded human prostate tissue stained for Ezrin using ab231907 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Ezrin antibody (ab231907)Anti-Ezrin antibody (ab231907) at 1 µg/ml + Recombinant human Ezrin protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
