Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14841] to EXOSC3 - BSA and Azide free
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free
See all EXOSC3 primary antibodies -
Description
Rabbit monoclonal [EPR14841] to EXOSC3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251025 is the carrier-free version of ab190689. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251025 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR14841 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : RAW264.7 cell line lysate
Lane 6 : NIH 3T3 cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaThis data was developed using ab190689, the same antibody clone in a different buffer formulation.
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This data was developed using ab190689, the same antibody clone in a different buffer formulation.
Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + PBS
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 30 kDa why is the actual band size different from the predicted?
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This data was developed using ab190689, the same antibody clone in a different buffer formulation.
Lane 1: Primary: ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
Lane 2: Primary: ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.
All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution
Lane 1 : Lysate of 293T cells immunoprecipated with ab190689 at 1/50
Lane 2 : PBS
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 30 kDa why is the actual band size different from the predicted?
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This data was developed using ab190689, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab190689, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaThis data was developed using ab190689, the same antibody clone in a different buffer formulation.
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Anti-EXOSC3 antibody [EPR14841] - C-terminal (ab190689) at 1/1000 dilution + 293T cell line lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaThis data was developed using ab190689, the same antibody clone in a different buffer formulation.
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