Anti-ETV2 antibody [EPR5229(3)] (ab181847)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5229(3)] to ETV2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ETV2 antibody [EPR5229(3)]
See all ETV2 primary antibodies -
Description
Rabbit monoclonal [EPR5229(3)] to ETV2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HUVEC cell lysate of lent-virally expressed Human ETV2 and Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2. HUVEC cells expressing exogenous Human ETV2.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5229(3) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ETV2 antibody [EPR5229(3)] (ab181847) at 1/10000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate of lent-virally expressed Human ETV2
Lane 3 : Human bone marrow stromal cell lysate
Lane 4 : Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
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