Anti-ETS2 antibody (ab272866)
Key features and details
- Rabbit polyclonal to ETS2
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ETS2 antibody
See all ETS2 primary antibodies -
Description
Rabbit polyclonal to ETS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human ETS2. The exact sequence is proprietary. Center region.
Database link: P15036 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Rat forebrain tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody (ab272866)
Paraffin-embedded rat forebrain tissue stained for ETS2 using ab272866 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody (ab272866)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ETS2 using ab272866 at 1/500 dilution (Green) in ICC/IF. Alpha-tubulin filaments (Red).
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Western blot - Anti-ETS2 antibody (ab272866)Anti-ETS2 antibody (ab272866) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS PAGE
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Immunoprecipitation - Anti-ETS2 antibody (ab272866)IP Sample: HeLa whole cell lysate/extract
7.5% SDS-PAGE
All lanes : Anti-ETS2 antibody (ab272866) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Control with 2 µg of preimmune rabbit IgG.
Lane 3 : Immunoprecipitation of ETS2 protein by 2 µg of ab272866
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : anti-rabbit IgG HRP