Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ETS2 antibody [EPR22419] (ab219948)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22419] to ETS2
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-ETS2 antibody [EPR22419]
See all ETS2 primary antibodies
Description

Rabbit monoclonal [EPR22419] to ETS2
Host species

Rabbit
Specificity

This antibody is not recommended for human in IHC.

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Mouse Rat
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: LPS treated RAW 264.7 cell lysate; K562 whole cell lysate; Human thymus lysate. IHC-P: Mouse and rat thymus tissue. ICC/IF: LPS treated RAW 264.7 cells. IP: K562 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22419
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat thymus (PMID: 3472202, PMID:26926107) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-ETS2 antibody [EPR22419] (ab219948)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling ETS2 with ab219948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (500 ng/ml) for 6 hours. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).
Secondary antibody contril: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Negative control: No staining on rat cerebellum (PMID: 3472202) is observed. Counterstained with hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS2 antibody [EPR22419] (ab219948)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ETS2 with ab219948 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Negative control: No staining on mouse cerebrum (PMID: 3472202) is observed. Counterstained with hematoxylin.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (ab219948)

ETS2 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate with ab219948 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219948 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: K562 whole cell lysate 10 µg (Input).
Lane 2: ab219948 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219948 in K562 whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.

The two immunoprecipitated bands represent non-phosphorylated and phosphorylated ETS2.
Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)

All lanes : Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 2 : Human thymus at 10 µg

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 26 seconds, Lane 2: 3 minutes.

Lane 1 was developed using a higher sensitivity ECL substrate.
Western blot - Anti-ETS2 antibody [EPR22419] (ab219948)

All lanes : Anti-ETS2 antibody [EPR22419] (ab219948) at 1/1000 dilution

Lane 1 : Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : RAW 264.7 treated with 500ng/ml lipopolysaccharides(LPS) for 6 hours

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 53 kDa
Observed band size: 50-55 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 15590657). The doublets represent ETS2 and its phosphorylated form.

This blot was developed using a higher sensitivity ECL substrate.
Anti-ETS2 antibody [EPR22419] (ab219948)

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