Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGS3 antibody (ab89726)

Key features and details

  • Rabbit polyclonal to AGS3
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-AGS3 antibody
    See all AGS3 primary antibodies

  • Description

    Rabbit polyclonal to AGS3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human AGS3 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90250)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HEK293; HepG2. IHC-P: human colon adenocarcinoma FFPE tissue sections

  • General notes

     This product was previously labelled as RNase H2 subunit C

     

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab89726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Cow
Application Abreviews Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).

Target

  • Relevance

    The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • AGS3 antibody
      • Aicardi-Goutieres syndrome 3 protein antibody
      • AYP1 antibody
      • Ribonuclease H2 subunit C antibody
      • Ribonuclease HI subunit C antibody
      • RNase H1 small subunit antibody
      • RNASEH2C antibody
      • RNH2C antibody
      see all

    Images

    • Western blot - Anti-AGS3 antibody (ab89726)
      Western blot - Anti-AGS3 antibody (ab89726)
      All lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 18 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 12 minutes


      AGS3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGS3 antibody (ab89726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGS3 antibody (ab89726)

      IHC image of AGS3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89726, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-AGS3 antibody (ab89726)
      Western blot - Anti-AGS3 antibody (ab89726)This image is courtesy of an Abreview submitted by Sarah Deasy
      All lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml

      All lanes : Mouse Pei1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 18 kDa
      Observed band size: 18 kDa


      Exposure time: 1 second


      Knockdown of Rnaseh2c using shRNA along with scramble control.

      Lane 1: scramble shRNA
      Lane 2: shRNA #1
      Lane 3: shRNA #2
      Lane 4: shRNA #3
      Lane 5: shRNA #4

      See Abreview

    References (3)

    Publishing research using ab89726? Please let us know so that we can cite the reference in this datasheet.

    ab89726 has been referenced in 3 publications.

    • Deasy SK  et al. Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer. PLoS Genet 15:e1008020 (2019). PubMed: 31125342
    • Shen T  et al. Antisense transcription regulates the expression of sense gene via alternative polyadenylation. Protein Cell 9:540-552 (2018). PubMed: 29273853
    • Uehara R  et al. Two RNase H2 Mutants with Differential rNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic Development. Cell Rep 25:1135-1145.e5 (2018). PubMed: 30380406

    Images

    • Western blot - Anti-AGS3 antibody (ab89726)
      Western blot - Anti-AGS3 antibody (ab89726)
      All lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 18 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 12 minutes


      AGS3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGS3 antibody (ab89726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGS3 antibody (ab89726)

      IHC image of AGS3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89726, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-AGS3 antibody (ab89726)
      Western blot - Anti-AGS3 antibody (ab89726) This image is courtesy of an Abreview submitted by Sarah Deasy
      All lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml

      All lanes : Mouse Pei1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 18 kDa
      Observed band size: 18 kDa


      Exposure time: 1 second


      Knockdown of Rnaseh2c using shRNA along with scramble control.

      Lane 1: scramble shRNA
      Lane 2: shRNA #1
      Lane 3: shRNA #2
      Lane 4: shRNA #3
      Lane 5: shRNA #4

      See Abreview

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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