Anti-AGS3 antibody (ab89726)
Key features and details
- Rabbit polyclonal to AGS3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AGS3 antibody
See all AGS3 primary antibodies -
Description
Rabbit polyclonal to AGS3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human AGS3 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90250) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HEK293; HepG2. IHC-P: human colon adenocarcinoma FFPE tissue sections
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General notes
This product was previously labelled as RNase H2 subunit C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications MouseCowApplication Abreviews Notes IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).Notes IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).Target
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Relevance
The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 84153 Human
- Entrez Gene: 68209 Mouse
- Omim: 610330 Human
- SwissProt: Q8TDP1 Human
- SwissProt: Q9CQ18 Mouse
- Unigene: 397010 Human
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Alternative names
- AGS3 antibody
- Aicardi-Goutieres syndrome 3 protein antibody
- AYP1 antibody
see all
Images
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Western blot - Anti-AGS3 antibody (ab89726)All lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesAGS3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGS3 antibody (ab89726)IHC image of AGS3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89726, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-AGS3 antibody (ab89726)This image is courtesy of an Abreview submitted by Sarah DeasyAll lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml
All lanes : Mouse Pei1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 1 secondKnockdown of Rnaseh2c using shRNA along with scramble control.
Lane 1: scramble shRNA
Lane 2: shRNA #1
Lane 3: shRNA #2
Lane 4: shRNA #3
Lane 5: shRNA #4
Protocols
Datasheets and documents
References (3)
ab89726 has been referenced in 3 publications.
- Deasy SK et al. Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer. PLoS Genet 15:e1008020 (2019). PubMed: 31125342
- Shen T et al. Antisense transcription regulates the expression of sense gene via alternative polyadenylation. Protein Cell 9:540-552 (2018). PubMed: 29273853
- Uehara R et al. Two RNase H2 Mutants with Differential rNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic Development. Cell Rep 25:1135-1145.e5 (2018). PubMed: 30380406
Images
-
Western blot - Anti-AGS3 antibody (ab89726)All lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesAGS3 contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGS3 antibody (ab89726)
IHC image of AGS3 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89726, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-AGS3 antibody (ab89726) This image is courtesy of an Abreview submitted by Sarah DeasyAll lanes : Anti-AGS3 antibody (ab89726) at 1 µg/ml
All lanes : Mouse Pei1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 1 secondKnockdown of Rnaseh2c using shRNA along with scramble control.
Lane 1: scramble shRNA
Lane 2: shRNA #1
Lane 3: shRNA #2
Lane 4: shRNA #3
Lane 5: shRNA #4
