Anti-ETFA antibody (ab126131)
Key features and details
- Rabbit polyclonal to ETFA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ETFA antibody
See all ETFA primary antibodies -
Description
Rabbit polyclonal to ETFA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-232 of Human ETFA
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Positive control
- A431 whole cell lysate, HeLa whole cell lysate, HeLa cells, Mouse C2C12 xenograft, Human colon carcinoma tissue
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General notes
Keep as a concentrated solutionReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ETFA antibody (ab126131) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : ETFA Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 35 kDa
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All lanes : Anti-ETFA antibody (ab126131) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
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ab126131, at 1/100 dilution (green), staining methanol fixed HeLa cells by immunofluorescence.
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ab126131, at 1/100 dilution, staining paraffin embedded Mouse C2C12 xenograft by immunohistochemistry.
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ab126131, at 1/000 dilution, staining paraffin embedded Human colon carcinoma by immunohistochemistry.