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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes Hect E3 Ligase

Anti-EDD antibody (ab175810)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-EDD antibody (ab175810)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to EDD
  • Suitable for: IP, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EDD antibody
    See all EDD primary antibodies
  • Description

    Rabbit polyclonal to EDD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human EDD aa 1900-2230.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Hect E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases

Images

  • Western blot - Anti-EDD antibody (ab175810)
    Western blot - Anti-EDD antibody (ab175810)
    All lanes : Anti-EDD antibody (ab175810)

    Lane 1 : Control si
    Lane 2 : UBR5 si
    Lane 3 : NIH 3T3 cell lysate at 40 µg
    Lane 4 : PC12 cell lysate at 40 µg

    Predicted band size: 309 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EDD antibody (ab175810)
    Immunocytochemistry/ Immunofluorescence - Anti-EDD antibody (ab175810)

    Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling EDD with ab175810 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. Top - EDD, Middle - DAPI, Bottom - Merge.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab175810)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDD antibody (ab175810)

    Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling EDD with ab175810 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Top - 4X, Middle - 10X, Bottom - 20X.

  • Immunoprecipitation - Anti-EDD antibody (ab175810)
    Immunoprecipitation - Anti-EDD antibody (ab175810)

    Immunoprecipitation analysis of U2OS cells labelling EED with ab175810. Protein A beads were crosslinked to the serum.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: WB

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