Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPESER1] to ESE1
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-ESE1 antibody [EPESER1]
See all ESE1 primary antibodies
Description

Rabbit monoclonal [EPESER1] to ESE1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human ESE1 aa 1 to the C-terminus.
Positive control
- Human uterus, fetal lung, A431, C6, Raw 264.7, PC-12, NIH3T3, and SKBR-3 cell lysates, A431 cells
General notes
This antibody was developed as part of a collaboration with the lab of Jim Hu at the University of Toronto.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPESER1
Isotype

IgG
Research areas

Flow Cytometry - Anti-ESE1 antibody [EPESER1] (ab133621)

Overlay histogram showing A431 cells stained with ab133621 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133621, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-ESE1 antibody [EPESER1] (ab133621)

All lanes : Anti-ESE1 antibody [EPESER1] (ab133621) at 1/1000 dilution

Lane 1 : Human uterus cell lysate
Lane 2 : Fetal lung cell lysate
Lane 3 : A431 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH3T3 cell lysate
Lane 8 : SKBR-3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa
Observed band size: 47 kDa
why is the actual band size different from the predicted?
Anti-ESE1 antibody [EPESER1] (ab133621)

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