Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ESE1 antibody [EPESER1] (ab133621)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPESER1] to ESE1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ESE1 antibody [EPESER1]
    See all ESE1 primary antibodies

  • Description

    Rabbit monoclonal [EPESER1] to ESE1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human ESE1 aa 1 to the C-terminus.

  • Positive control

    • Human uterus, fetal lung, A431, C6, Raw 264.7, PC-12, NIH3T3, and SKBR-3 cell lysates, A431 cells

  • General notes

    This antibody was developed as part of a collaboration with the lab of Jim Hu at the University of Toronto.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPESER1

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-ESE1 antibody [EPESER1] (ab133621)
    Flow Cytometry - Anti-ESE1 antibody [EPESER1] (ab133621)
    Overlay histogram showing A431 cells stained with ab133621 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133621, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-ESE1 antibody [EPESER1] (ab133621)
    Western blot - Anti-ESE1 antibody [EPESER1] (ab133621)
    All lanes : Anti-ESE1 antibody [EPESER1] (ab133621) at 1/1000 dilution

    Lane 1 : Human uterus cell lysate
    Lane 2 : Fetal lung cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : C6 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : PC12 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : SKBR-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?

  • Anti-ESE1 antibody [EPESER1] (ab133621)
    Anti-ESE1 antibody [EPESER1] (ab133621)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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