Anti-ESCO2 antibody (ab86003)
Key features and details
- Rabbit polyclonal to ESCO2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ESCO2 antibody
See all ESCO2 primary antibodies -
Description
Rabbit polyclonal to ESCO2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A synthetic peptide corresponding to a region between residue 200 and 250 of human ESCO2 (NP_001017420.1).
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Positive control
- HeLa whole cell lysate (ab150035).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab86003 was affinity purified using an epitope specific to ESCO2 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ESCO2 antibody (ab86003)All lanes : Anti-ESCO2 antibody (ab86003) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ESCO2 antibody (ab86003)Detection of human ESCO2 by immunoprecipitation. 1mg HeLa Detection of ESCO2 by Western Blot of Immunprecipitate. ab86003 at 1µg/ml staining ESCO2 in HeLa whole cell lysate immunoprecipitated using ab86003 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
