Anti-ERK1 antibody (ab264269)
Key features and details
- Rabbit polyclonal to ERK1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 antibody
See all ERK1 primary antibodies -
Description
Rabbit polyclonal to ERK1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide aa 329-379. The exact sequence is proprietary. NP_002737.2
Database link: P27361 -
Positive control
- IHC-P:human breast carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa and Hek-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERK1 antibody (ab264269)All lanes : Anti-ERK1 antibody (ab264269) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Hek293-T whole cell lysate at 50 µgDetected with chemilimuniscence with exposure time of 30 seconds.
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Immunoprecipitation - Anti-ERK1 antibody (ab264269)ERK1was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264269 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using ab264269 at 1 μg/ml.
Lane 1: ab264269 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 antibody (ab264269)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for ERK1 using ab264269 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
