Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERH antibody [EPR10830(B)] (ab166620)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10830(B)] to ERH
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ERH antibody [EPR10830(B)]
    See all ERH primary antibodies

  • Description

    Rabbit monoclonal [EPR10830(B)] to ERH

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human ERH (P84090).

  • Positive control

    • T47-D, SH-SY5Y, A549 and A431 cell lysates, Human cervical carcinoma and prostate tissue, A431 cells, immunoprecipitation pellet from A549 cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10830(B)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (ab166620)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling ERH with ab166620 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Western blot - Anti-ERH antibody [EPR10830(B)] (ab166620)
    All lanes : Anti-ERH antibody [EPR10830(B)] (ab166620) at 1/1000 dilution

    Lane 1 : T47-D cell lysates
    Lane 2 : SH-SY5Y cell lysates
    Lane 3 : A549 cell lysates
    Lane 4 : A431 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 12 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (ab166620)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ERH with ab166620 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Immunofluorescence analysis of A431 cells labeling ERH with ab166620 at 1/250 dilution.
  • Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] (ab166620)
    Immunoprecipitation analysis of ERH in pellet from A549 cell lysate using ab166620 at 1/10 dilution, followed by WB detection.

    Anti-ERH antibody [EPR10830(B)] (ab166620) at 1/1000 dilution + immunoprecipitation pellet from A549 cell lysate at 10 µg
  • Anti-ERH antibody [EPR10830(B)] (ab166620)
    Anti-ERH antibody [EPR10830(B)] (ab166620)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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