Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eRF3/GSPT1 + eRF3B/GSPT2 antibody [EPR22908-63] - BSA and Azide free (ab256479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22908-63] to eRF3/GSPT1 + eRF3B/GSPT2 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eRF3/GSPT1 + eRF3B/GSPT2 antibody [EPR22908-63] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22908-63] to eRF3/GSPT1 + eRF3B/GSPT2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-3, HeLa, Rat kidney, His tagged human eRF3a recombinant protein, His tagged human eRF3b recombinant protein, PC-12 and NIH/3T3 lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa and NIH/3T3 cells. IP: PC-3 cells.

  • General notes

    Ab256479 is the carrier-free version of ab256471. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256479 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)
    Immunoprecipitation - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)

    eRF3a+eRF3b was immunoprecipitated from 0.35 mg PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate with ab256471 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256471. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate 10µg

    Lane 2: ab256471 IP in PC-3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256471 in PC-3 whole cell lysate.

    Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256471).

  • Flow Cytometry - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)
    Flow Cytometry - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling eRF3a+eRF3b with ab256471 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256471).

  • Flow Cytometry - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)
    Flow Cytometry - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling eRF3a+eRF3b with ab256471 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256471).

  • Immunocytochemistry/ Immunofluorescence - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)
    Immunocytochemistry/ Immunofluorescence - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling eRF3a+eRF3b with ab256471 at 1/1000 (5 ug/ml) dilution, followed by anti- eRF3a+eRF3b ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256471).

  • Immunocytochemistry/ Immunofluorescence - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)
    Immunocytochemistry/ Immunofluorescence - Anti-eRF3/GSPT1 antibody [EPR22908-63] - BSA and Azide free (ab256479)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling eRF3a+eRF3b with ab256471 at 1/1000 (5 ug/ml) dilution, followed by anti- eRF3a+eRF3b ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256471).

  • Anti-eRF3/GSPT1 + eRF3B/GSPT2 antibody [EPR22908-63] - BSA and Azide free (ab256479)
    Anti-eRF3/GSPT1 + eRF3B/GSPT2 antibody [EPR22908-63] - BSA and Azide free (ab256479)

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