Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)
![Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)](https://www.abcam.com/ps/products/240/ab240096/Images/ab240096-403389-anti-ercc8-antibody-epr9237-bsa-and-azide-free-western-blot.jpg "Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9237] to ERCC8 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ERCC8 antibody [EPR9237] - BSA and Azide free
See all ERCC8 primary antibodies -
Description
Rabbit monoclonal [EPR9237] to ERCC8 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, HeLa, and MOLT-4; IP: HeLa whole cell lysate.
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General notes
ab240096 is the carrier-free version of ab137033 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab240096 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9237 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)
This data was developed using ab137033, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ERCC8 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Molt-4 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab137033 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab137033 was shown to recognize ERCC8 when ERCC8 knockout samples were used, along with additional cross-reactive bands. Wild-type and ERCC8 knockout samples were subjected to SDS-PAGE. ab137033 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)](https://www.abcam.com/ps/products/240/ab240096/Images/ab240096-4-anti-ercc8-antibody-epr9237-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)This data was developed using ab137033, the same antibody clone in a different buffer formulation.
Purified ab137033 at 1/80 dilution (2µg) immunoprecipitating ERCC8 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab137033 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 44 kDa -
![Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)](https://www.abcam.com/ps/products/240/ab240096/Images/ab240096-3-benefits-of-recombinant-antibodies.png)
Anti-ERCC8 antibody [EPR9237] - BSA and Azide free (ab240096)
