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Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free
    See all ErbB2 / HER2 primary antibodies
  • Description

    Rabbit monoclonal [EP1046Y] to ErbB2 / HER2 (phospho Y1139) - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SKBR3 and EGF cell lysate.
  • General notes

    ab182447 is the carrier-free version of ab53290 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab182447 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1046Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Images

  • Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/500 dilution

    Lane 1 : A431 whole cell lysate - untreated
    Lane 2 : A431 whole cell lysate - treated with Epidermal Growth Factor (EGF)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 138 kDa
    Observed band size: 185 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab53290, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    Immunoprecipitation - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)

    This data was developed using ab53290&

    44; the same antibody clone in a different buffer formulation.

    >ab53290 at 1/70 dilution immunoprecipitating ErbB2 / HER2 (phospho Y1139) in A431 (human epidermoid carcinoma) whole cell lysate.

    Lane 1 (input): A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate 10µg

    Lane 2 (+): ab53290 + A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53290 in A431 treated with 100 ng/mL EGF for 10 minutes whole cell lysate

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    Dot Blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)

    This data was developed using ab53290, the same antibody clone in a different buffer formulation.Dot blot analysis of ErbB2 / HER2 (pY1139) peptide (Lane 1) and ErbB2 / HER2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y1139) with ab53290 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.

  • Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    Western blot - Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    All lanes : Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] (ab53290) at 1/1000 dilution

    Lane 1 : SKBR3 cell lysate
    Lane 2 : SKBR3 cell lysate - treated with EGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 138 kDa
    Observed band size: 185 kDa why is the actual band size different from the predicted?



    This data was developed using ab53290, the same antibody clone in a different buffer formulation.

  • Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)
    Anti-ErbB2 / HER2 (phospho Y1139) antibody [EP1046Y] - BSA and Azide free (ab182447)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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