Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)
![Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)](https://www.abcam.com/ps/products/264/ab264541/Images/ab264541-1-anti-erbb-2-antibody-h2mab139-immunohistochemistry-human-breast-carcinoma.jpg "Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [H2Mab-139] to ErbB2 / HER2
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 antibody [H2Mab-139]
See all ErbB2 / HER2 primary antibodies -
Description
Mouse monoclonal [H2Mab-139] to ErbB2 / HER2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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Immunogen
Recombinant fragment corresponding to Human ErbB2/ HER2.
Database link: P04626 -
Positive control
- IHC-P: Human breast carcinoma tissue. Flow Cyt: HT-29 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
H2Mab-139 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab264541 at 1/100 dilution followed by a ready to use secondary antibody. Membranous staining on human breast carcinoma is observed. The section was incubated with ab264541 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use secondary antibody.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)](https://www.abcam.com/ps/products/264/ab264541/Images/ab264541-2-anti-erbb-2-antibody-h2mab139-immunohistochemistry-human-breast-carcinoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab264541 at 1/100 dilution followed by a ready to use secondary antibody. No staining on human breast carcinoma without expression of HER2 is observed. The section was incubated with ab264541 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use secondary antibody.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Flow Cytometry - Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)](https://www.abcam.com/ps/products/264/ab264541/Images/ab264541-3-anti-erbb-2-antibody-h2mab139-flow-cytometry-ht29.jpg)
Flow Cytometry - Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)Flow cytometric analysis of HT-29 (human colorectal adenocarcinoma epithelial cell) cells labelling ErbB2 / HER2 with ab264541 at 1/500 dilution (0.1µg) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)](https://www.abcam.com/ps/products/264/ab264541/Images/ab264541-4-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [H2Mab-139] (ab264541)