Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
Key features and details
- Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody
See all Eph receptor B1 + Eph receptor B2 primary antibodies -
Description
Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) -
Host species
Rabbit -
Specificity
ab61791 detects endogenous levels of EPHB1/2 only when phosphorylated at tyrosine 594 and tyrosine 596.
-
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Eph receptor B1 + Eph receptor B2.
-
Positive control
- Extracts from HepG2 cells, HuvEc cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate - overexpressing EphB2
Lane 3 : HeLa whole cell lysate - overexpressinf EphB2-kinase dead (K661R)
Lysates/proteins at 100000 cells per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutesAll cells were stimulated with preclustered ephrinb1-Fc chimeric protein.
-
ab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.
-
All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with the immunising phosphopeptide
Predicted band size: 110 kDa
Observed band size: 110 kDa
-
Immunofluorescence analysis of HuvEc cells, using ab61791 at 1/500-1/1000 dilution with (+) or without (-) immunising phosphopeptide.