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Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody
    See all Eph receptor B1 + Eph receptor B2 primary antibodies
  • Description

    Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596)
  • Host species

    Rabbit
  • Specificity

    ab61791 detects endogenous levels of EPHB1/2 only when phosphorylated at tyrosine 594 and tyrosine 596.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Eph receptor B1 + Eph receptor B2.

  • Positive control

    • Extracts from HepG2 cells, HuvEc cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Ephrins
    • Eph Receptors
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors

Images

  • Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
    Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) This image is courtesy of an Abreview submitted by Thomas Jungas
    All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate - overexpressing EphB2
    Lane 3 : HeLa whole cell lysate - overexpressinf EphB2-kinase dead (K661R)

    Lysates/proteins at 100000 cells per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 110 kDa
    Observed band size: 110 kDa


    Exposure time: 5 minutes


    All cells were stimulated with preclustered ephrinb1-Fc chimeric protein.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) This image is courtesy of an Abreview submitted by Thomas Jungas

    ab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing  EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.

    See Abreview

  • Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
    Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
    All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with the immunising phosphopeptide

    Predicted band size: 110 kDa
    Observed band size: 110 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)
    Immunofluorescence analysis of HuvEc cells, using ab61791 at 1/500-1/1000 dilution with (+) or without (-) immunising phosphopeptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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