Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24479-136] to Eph receptor A3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free
    See all Eph receptor A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR24479-136] to Eph receptor A3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 22Rv1 and B16-F10 untreated/treated with PNGase F whole cell lysate. His-tagged human EPHA3 recombinant protein.

  • General notes

    ab281946 is the carrier-free version of ab281926. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24479-136

  • Isotype

    IgG

Images

  • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

    Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
    Lane 2 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 110 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?



    This data was developed using ab281926, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HL-60 (PMID: 18814179).

    Exposure time: 59 seconds.

  • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

    Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
    Lane 2 : 22Rv1 whole cell lysate treated with PNGase F
    Lane 3 : B16-F10 (Mouse skin melanoma), whole cell lysate
    Lane 4 : B16-F10 whole cell lysate treated with PNGase F

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 110 kDa
    Observed band size: 140-150 kDa why is the actual band size different from the predicted?



    This data was developed using ab281926, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    EPHA3 is a glycoprotein and can be de-glycosylated by PNGase F.

    The molecular mass observed is consistent with the literature (PMID:28415715).

    Exposure times: Lane 1-2: 37 secs; Lane 3-4: 15 secs.

  • Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution

    Lane 1 : His-tagged human EPHA3 recombinant protein at 0.01 µg
    Lane 2 : His-tagged human EPHA2 recombinant protein at 0.01 µg
    Lane 3 : His-tagged human EPHA4 recombinant protein at 0.01 µg
    Lane 4 : His-tagged human EPHA7 recombinant protein at 0.01 µg
    Lane 5 : His-tagged human EPHA5 recombinant protein at 0.1 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 110 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab281926, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 5.5 secs; Lane 5: 26 secs.

  • Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
    Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)

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