Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
![Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)](https://www.abcam.com/ps/products/281/ab281946/Images/ab281946-459970-anti-eph-receptor-a3-antibody-epr24479136-western-blot-carcinoma.jpg "Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24479-136] to Eph receptor A3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free
See all Eph receptor A3 primary antibodies -
Description
Rabbit monoclonal [EPR24479-136] to Eph receptor A3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 22Rv1 and B16-F10 untreated/treated with PNGase F whole cell lysate. His-tagged human EPHA3 recombinant protein.
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General notes
ab281946 is the carrier-free version of ab281926. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab281946 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24479-136 -
Isotype
IgG
Images
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Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution
Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 2 : HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 110 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab281926, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HL-60 (PMID: 18814179).
Exposure time: 59 seconds.
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![Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)](https://www.abcam.com/ps/products/281/ab281946/Images/ab281946-459971-anti-eph-receptor-a3-antibody-epr24479136-western-blot-carcinoma1.jpg)
Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution
Lane 1 : 22Rv1 (human prostate carcinoma epithelial cell), whole cell lysate
Lane 2 : 22Rv1 whole cell lysate treated with PNGase F
Lane 3 : B16-F10 (Mouse skin melanoma), whole cell lysate
Lane 4 : B16-F10 whole cell lysate treated with PNGase F
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 140-150 kDa why is the actual band size different from the predicted?This data was developed using ab281926, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
EPHA3 is a glycoprotein and can be de-glycosylated by PNGase F.
The molecular mass observed is consistent with the literature (PMID:28415715).
Exposure times: Lane 1-2: 37 secs; Lane 3-4: 15 secs.
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![Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)](https://www.abcam.com/ps/products/281/ab281946/Images/ab281946-460009-anti-eph-receptor-a3-antibody-epr24479136-western-blot-recombinant.jpg)
Western blot - Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)All lanes : Anti-Eph receptor A3 antibody [EPR24479-136] (ab281926) at 1/1000 dilution
Lane 1 : His-tagged human EPHA3 recombinant protein at 0.01 µg
Lane 2 : His-tagged human EPHA2 recombinant protein at 0.01 µg
Lane 3 : His-tagged human EPHA4 recombinant protein at 0.01 µg
Lane 4 : His-tagged human EPHA7 recombinant protein at 0.01 µg
Lane 5 : His-tagged human EPHA5 recombinant protein at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?This data was developed using ab281926, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lanes 1-4: 5.5 secs; Lane 5: 26 secs.
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![Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)](https://www.abcam.com/ps/products/281/ab281946/Images/ab281946-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Eph receptor A3 antibody [EPR24479-136] - BSA and Azide free (ab281946)
