Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)
![Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)](https://www.abcam.com/ps/products/250/ab250998/Images/ab250998-408229-anti-eno1-eno2-eno3-antibody-epr18422-bsa-and-azide-free-western-blot.jpg "Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18422] to ENO1 + ENO2 + ENO3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free
See all ENO1 + ENO2 + ENO3 primary antibodies -
Description
Rabbit monoclonal [EPR18422] to ENO1 + ENO2 + ENO3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250998 is the carrier-free version of ab189892. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250998 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR18422 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lane 1 : Human ENO1 full length recombinant protein
Lane 2 : Human ENO2 full length recombinant protein
Lane 3 : Human ENO3 full length recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsThis data was developed using ab189892, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human ENO1 full length recombinant protein (Cat no.: ab89248) containing aa1-434.
Human ENO2 full length recombinant protein containing aa1-434 with a His-Tag®.
Human ENO3 full length recombinant protein (Cat no.: ab113127) containing aa1-434 with a His-Tag®.
Human ENO2 full length recombinant protein was made in-house.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)](https://www.abcam.com/ps/products/250/ab250998/Images/ab250998-405423-anti-eno1-eno2-eno3-antibody-epr18422-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : Human fetal spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsThis data was developed using ab189892, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-3:5 seconds; Lane 4: 30 seconds.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)](https://www.abcam.com/ps/products/250/ab250998/Images/ab250998-403247-anti-eno1-eno2-eno3-antibody-epr18422-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 secondsThis data was developed using ab189892, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)](https://www.abcam.com/ps/products/250/ab250998/Images/ab250998-399381-anti-eno1-eno2-eno3-antibody-epr18422-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)Lanes 1-7 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lanes 8-10 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 secondThis data was developed using ab189892, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4 and 8-10: 1 second; Lanes 5-7: 3 seconds.
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![Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)](https://www.abcam.com/ps/products/250/ab250998/Images/ab250998-5-benefits-of-recombinant-antibodies.png)
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] - BSA and Azide free (ab250998)