Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Acid sphingomyelinase antibody [EPR23090-181] - BSA and Azide free (ab273510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23090-181] to Acid sphingomyelinase - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Acid sphingomyelinase antibody [EPR23090-181] - BSA and Azide free
    See all Acid sphingomyelinase primary antibodies

  • Description

    Rabbit monoclonal [EPR23090-181] to Acid sphingomyelinase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 treated with 80nM phorbol-12-myristate-13-acetate and HepG2 whole cell lysates; Human heart tissue lysate.

  • General notes

    ab273510 is the carrier-free version of ab272729. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Acid sphingomyelinase antibody [EPR23090-181] - BSA and Azide free (ab273510)
    Western blot - Anti-Acid sphingomyelinase antibody [EPR23090-181] - BSA and Azide free (ab273510)
    Anti-Acid sphingomyelinase antibody [EPR23090-181] (ab272729) at 1/500 dilution + Human heart tissue lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    This data was developed using the same  antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab272729).

  • Anti-Acid sphingomyelinase antibody [EPR23090-181] - BSA and Azide free (ab273510)
    Anti-Acid sphingomyelinase antibody [EPR23090-181] - BSA and Azide free (ab273510)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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