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Anti-ELOVL4 antibody (ab224608)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-ELOVL4 antibody (ab224608)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ELOVL4
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ELOVL4 antibody
    See all ELOVL4 primary antibodies
  • Description

    Rabbit polyclonal to ELOVL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Recombinant fragment corresponding to Human ELOVL4 aa 250 to the C-terminus.
    Database link: Q9GZR5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HepG2 cell lysate; rat heart and liver tissue lysate. IHC: Human testis and small intestine tissue. ICC/IF: HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Visual system
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Images

  • Western blot - Anti-ELOVL4 antibody (ab224608)
    Western blot - Anti-ELOVL4 antibody (ab224608)
    All lanes : Anti-ELOVL4 antibody (ab224608) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat liver lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Blocking: 5% milk (non-fat dry milk dissolved in PBST) incubate overnight under 4 oC.
    Primary antibody incubation: Dilute primary antibody with 5% milk (non-fat dry milk dissolved in PBST), incubate overnight at 4oC.
    Washing: PBST, 4 times, 10 minutes.
    Secondary antibody incubation: Dilute secondary antibody at 1/10000 or 1/20000 with 5% milk (non-fat dry milk dissolved in PBST), incubate for 1 hour under 25oC.
    Washing: PBST, 4 times, 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608)

    Paraffin-embedded human testis tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL4 antibody (ab224608)

    Paraffin-embedded human small intestine tissue stained for ELOVL4 using ab224608 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (ab224608)
    Immunocytochemistry/ Immunofluorescence - Anti-ELOVL4 antibody (ab224608)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ELOVL4 (green) using ab224608 at 1/100 dilution in ICC/IF.

    Secondary antibody: Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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