Anti-ABCB6/PRP antibody [EPR21891] (ab221159)
![Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.com/ps/products/221/ab221159/Images/ab221159-312403-anti-abcb6-antibody-epr21891-flow-cytometry.jpg "Anti-ABCB6/PRP antibody [EPR21891] (ab221159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21891] to ABCB6/PRP
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ABCB6/PRP antibody [EPR21891]
See all ABCB6/PRP primary antibodies -
Description
Rabbit monoclonal [EPR21891] to ABCB6/PRP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa and K562 cells. Flow: HeLa cells.
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General notes
This product was previously labelled as ABCB6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21891 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ABCB6/PRP with ab221159 at 1/500 dilution (red)compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.com/ps/products/221/ab221159/Images/ab221159-312402-anti-abcb6-antibody-epr21891-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling ABCB6/PRP with ab221159 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing lysosome staining in K-562 cell line.
The nuclear counter stain is DAPI (blue). Counterstained with ab25630 anti-LAMP1 (mouse mAb) at 100 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.com/ps/products/221/ab221159/Images/ab221159-312401-anti-abcb6-antibody-epr21891-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ABCB6/PRP with ab221159 at 1/500 dilution, followed by ab150077 AlexaFluorGoat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing lysosome staining in K-562 cell line.
The nuclear counter stain is DAPI (blue). Counterstained with ab25630 anti-LAMP1 (mouse mAb) at 100 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.com/ps/products/221/ab221159/Images/ab221159-4-benefits-of-recombinant-antibodies.png)
Anti-ABCB6/PRP antibody [EPR21891] (ab221159)
